normalized rna-seq data Search Results


hiseq rsem gene-normalized rna-seq data  (Illumina Inc)
90
Illumina Inc hiseq rsem gene-normalized rna-seq data
Hiseq Rsem Gene Normalized Rna Seq Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tpm-normalized rna-seq expression data of gapdhs  (Human Protein Atlas)
90
Human Protein Atlas tpm-normalized rna-seq expression data of gapdhs
Tpm Normalized Rna Seq Expression Data Of Gapdhs, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normalized rna-seq data of pdcls  (Brunton Inc)
90
Brunton Inc normalized rna-seq data of pdcls
( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary <t>PDCLs</t> were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based <t>on</t> <t>RNA-Seq</t> in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.
Normalized Rna Seq Data Of Pdcls, supplied by Brunton Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna-seq data from five normal skin samples  (ProteoGenex Inc)
90
ProteoGenex Inc rna-seq data from five normal skin samples
( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary <t>PDCLs</t> were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based <t>on</t> <t>RNA-Seq</t> in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.
Rna Seq Data From Five Normal Skin Samples, supplied by ProteoGenex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normalized rna-seq data  (Biotechnology Information)
90
Biotechnology Information normalized rna-seq data
( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary <t>PDCLs</t> were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based <t>on</t> <t>RNA-Seq</t> in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.
Normalized Rna Seq Data, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna-seq data rsem upperquartile-normalized mrna data  (Illumina Inc)
90
Illumina Inc rna-seq data rsem upperquartile-normalized mrna data
( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary <t>PDCLs</t> were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based <t>on</t> <t>RNA-Seq</t> in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.
Rna Seq Data Rsem Upperquartile Normalized Mrna Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnaseq polya + illumina pancan normalized data  (Illumina Inc)
90
Illumina Inc rnaseq polya + illumina pancan normalized data
( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary <t>PDCLs</t> were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based <t>on</t> <t>RNA-Seq</t> in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.
Rnaseq Polya + Illumina Pancan Normalized Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna-seq data for a normal breast epithelial cell line  (Biotechnology Information)
90
Biotechnology Information rna-seq data for a normal breast epithelial cell line
( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary <t>PDCLs</t> were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based <t>on</t> <t>RNA-Seq</t> in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.
Rna Seq Data For A Normal Breast Epithelial Cell Line, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normalized rna-seq data tcga v2  (MedCalc Software Ltd)
90
MedCalc Software Ltd normalized rna-seq data tcga v2
( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary <t>PDCLs</t> were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based <t>on</t> <t>RNA-Seq</t> in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.
Normalized Rna Seq Data Tcga V2, supplied by MedCalc Software Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal ovary tissue rna-seq data set  (Illumina Inc)
90
Illumina Inc normal ovary tissue rna-seq data set
( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary <t>PDCLs</t> were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based <t>on</t> <t>RNA-Seq</t> in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.
Normal Ovary Tissue Rna Seq Data Set, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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poly(a)+ rna-seq data for normal healthy tissues  (Broad Institute Inc)
90
Broad Institute Inc poly(a)+ rna-seq data for normal healthy tissues
( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary <t>PDCLs</t> were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based <t>on</t> <t>RNA-Seq</t> in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.
Poly(A)+ Rna Seq Data For Normal Healthy Tissues, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tcga rsem normalized rna-seq gene expression data illumina hiseq  (Illumina Inc)
90
Illumina Inc tcga rsem normalized rna-seq gene expression data illumina hiseq
Hazard Ratio and P value of each group.
Tcga Rsem Normalized Rna Seq Gene Expression Data Illumina Hiseq, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary PDCLs were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based on RNA-Seq in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.

Journal: JCI Insight

Article Title: Epigenetic drug screening defines a PRMT5 inhibitor–sensitive pancreatic cancer subtype

doi: 10.1172/jci.insight.151353

Figure Lengend Snippet: ( A ) Dose-response curves of 4 human PDOs after 6 days of treatment with JNJ-64619178. Viability was determined with CellTiter-Glo assay. Sensitive: red, resistant: blue. ( B ) Microscopy of a sensitive organoid treated with the indicated dose of JNJ-64619178 over 6 days. Scale bar: 500 μM. ( C ) GI 50 values of 24 human PDOs were calculated. Sensitive: red, resistant: blue. ( D ) MYC expression was analyzed in selected PDOs ( n = 7) by Western blot. β-Actin: loading control. Sensitive: red, resistant: blue. One Western blot was performed. ( E ) GI 50 values of 18 primary PDCLs were calculated. Sensitive: red, resistant: blue. ( F ) Relative MYC protein expression, determined by Western blot, in sensitive ( n = 3, red) and resistant ( n = 15, blue) PDCL was compared. P value of Mann-Whitney U test is depicted. MYC Western blot was performed once. ( G ) MYC mRNA expression based on RNA-Seq in JNJ-64619178–sensitive and –resistant PDCLs. * P < 0.05; Mann-Whitney U test. ( H ) JNJ-64619178 GI 50 values of PDOs were grouped into quartiles and differentially expressed genes of most sensitive (first quartile, n = 6) and most resistant (fourth quartile, n = 6) PDOs were calculated. The log-fold change was used as a rank to perform a preranked GSEA. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( I ) JNJ-64619178 GI 50 values of PDCL were grouped into quartiles and analyzed corresponding to H . Sensitive: first quartile, n = 3, resistant: fourth quartile, n = 3. Depicted are the top 10 HALLMARK signatures; q value is color coded. ( J ) Proteomics-based protein expression of PRMT5i-sensitive (first quartile, n = 6) and -resistant (fourth quartile, n = 6) PDOs was used to determine differentially expressed proteins. All proteins upregulated in sensitive PDOs were analyzed using the Enrichr web tool. Combined scores of the HALLMARK signatures with an adjusted P value and q < 0.05 are shown.

Article Snippet: Normalized RNA-Seq data of PDCLs were obtained from the supplementary information of Brunton and colleagues ( ).

Techniques: Glo Assay, Microscopy, Expressing, Western Blot, Control, MANN-WHITNEY, RNA Sequencing

Hazard Ratio and P value of each group.

Journal: PeerJ

Article Title: Immune cell landscape analysis reveals prognostic immune cells and its potential mechanism in squamous cell lung carcinoma

doi: 10.7717/peerj.9996

Figure Lengend Snippet: Hazard Ratio and P value of each group.

Article Snippet: Secondly, the clinical information, including age, gender, TNM stage, and smoking history , together with TCGA RSEM normalized RNA-seq gene expression data (Illumina HiSeq) of 490 LUSC patients were obtained from UCSC Xena ( https://xena.ucsc.edu/public ).

Techniques: